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Null Hypothesis Examples

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The null hypothesis —which assumes that there is no meaningful relationship between two variables—may be the most valuable hypothesis for the scientific method because it is the easiest to test using a statistical analysis. This means you can support your hypothesis with a high level of confidence. Testing the null hypothesis can tell you whether your results are due to the effect of manipulating ​ the dependent variable or due to chance.

What Is the Null Hypothesis?

The null hypothesis states there is no relationship between the measured phenomenon (the dependent variable) and the independent variable . You do not​ need to believe that the null hypothesis is true to test it. On the contrary, you will likely suspect that there is a relationship between a set of variables. One way to prove that this is the case is to reject the null hypothesis. Rejecting a hypothesis does not mean an experiment was "bad" or that it didn't produce results. In fact, it is often one of the first steps toward further inquiry.

To distinguish it from other hypotheses, the null hypothesis is written as ​ H 0  (which is read as “H-nought,” "H-null," or "H-zero"). A significance test is used to determine the likelihood that the results supporting the null hypothesis are not due to chance. A confidence level of 95 percent or 99 percent is common. Keep in mind, even if the confidence level is high, there is still a small chance the null hypothesis is not true, perhaps because the experimenter did not account for a critical factor or because of chance. This is one reason why it's important to repeat experiments.

Examples of the Null Hypothesis

To write a null hypothesis, first start by asking a question. Rephrase that question in a form that assumes no relationship between the variables. In other words, assume a treatment has no effect. Write your hypothesis in a way that reflects this.

• What Is a Hypothesis? (Science)
• What 'Fail to Reject' Means in a Hypothesis Test
• What Are the Elements of a Good Hypothesis?
• Scientific Method Vocabulary Terms
• Null Hypothesis Definition and Examples
• Definition of a Hypothesis
• Six Steps of the Scientific Method
• Understanding Simple vs Controlled Experiments
• Hypothesis Test for the Difference of Two Population Proportions
• What Is the Difference Between Alpha and P-Values?
• Null Hypothesis and Alternative Hypothesis
• What Are Examples of a Hypothesis?
• Hypothesis Test Example
• How to Conduct a Hypothesis Test
• What Is a P-Value?
• The Difference Between Type I and Type II Errors in Hypothesis Testing

Statistics Made Easy

How to Write a Null Hypothesis (5 Examples)

A hypothesis test uses sample data to determine whether or not some claim about a population parameter is true.

Whenever we perform a hypothesis test, we always write a null hypothesis and an alternative hypothesis, which take the following forms:

H 0 (Null Hypothesis): Population parameter =,  ≤, ≥ some value

H A  (Alternative Hypothesis): Population parameter <, >, ≠ some value

Note that the null hypothesis always contains the equal sign .

We interpret the hypotheses as follows:

Null hypothesis: The sample data provides no evidence to support some claim being made by an individual.

Alternative hypothesis: The sample data  does provide sufficient evidence to support the claim being made by an individual.

For example, suppose it’s assumed that the average height of a certain species of plant is 20 inches tall. However, one botanist claims the true average height is greater than 20 inches.

To test this claim, she may go out and collect a random sample of plants. She can then use this sample data to perform a hypothesis test using the following two hypotheses:

H 0 : μ ≤ 20 (the true mean height of plants is equal to or even less than 20 inches)

H A : μ > 20 (the true mean height of plants is greater than 20 inches)

If the sample data gathered by the botanist shows that the mean height of this species of plants is significantly greater than 20 inches, she can reject the null hypothesis and conclude that the mean height is greater than 20 inches.

Read through the following examples to gain a better understanding of how to write a null hypothesis in different situations.

Example 1: Weight of Turtles

A biologist wants to test whether or not the true mean weight of a certain species of turtles is 300 pounds. To test this, he goes out and measures the weight of a random sample of 40 turtles.

Here is how to write the null and alternative hypotheses for this scenario:

H 0 : μ = 300 (the true mean weight is equal to 300 pounds)

H A : μ ≠ 300 (the true mean weight is not equal to 300 pounds)

Example 2: Height of Males

It’s assumed that the mean height of males in a certain city is 68 inches. However, an independent researcher believes the true mean height is greater than 68 inches. To test this, he goes out and collects the height of 50 males in the city.

H 0 : μ ≤ 68 (the true mean height is equal to or even less than 68 inches)

H A : μ > 68 (the true mean height is greater than 68 inches)

Example 3: Graduation Rates

A university states that 80% of all students graduate on time. However, an independent researcher believes that less than 80% of all students graduate on time. To test this, she collects data on the proportion of students who graduated on time last year at the university.

H 0 : p ≥ 0.80 (the true proportion of students who graduate on time is 80% or higher)

H A : μ < 0.80 (the true proportion of students who graduate on time is less than 80%)

Example 4: Burger Weights

A food researcher wants to test whether or not the true mean weight of a burger at a certain restaurant is 7 ounces. To test this, he goes out and measures the weight of a random sample of 20 burgers from this restaurant.

H 0 : μ = 7 (the true mean weight is equal to 7 ounces)

H A : μ ≠ 7 (the true mean weight is not equal to 7 ounces)

Example 5: Citizen Support

A politician claims that less than 30% of citizens in a certain town support a certain law. To test this, he goes out and surveys 200 citizens on whether or not they support the law.

H 0 : p ≥ .30 (the true proportion of citizens who support the law is greater than or equal to 30%)

H A : μ < 0.30 (the true proportion of citizens who support the law is less than 30%)

Additional Resources

Introduction to Hypothesis Testing Introduction to Confidence Intervals An Explanation of P-Values and Statistical Significance

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• Knowledge Base
• Null and Alternative Hypotheses | Definitions & Examples

Null and Alternative Hypotheses | Definitions & Examples

Published on 5 October 2022 by Shaun Turney . Revised on 6 December 2022.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

• Null hypothesis (H 0 ): There’s no effect in the population .
• Alternative hypothesis (H A ): There’s an effect in the population.

The effect is usually the effect of the independent variable on the dependent variable .

Table of contents

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, differences between null and alternative hypotheses, how to write null and alternative hypotheses, frequently asked questions about null and alternative hypotheses.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”, the null hypothesis (H 0 ) answers “No, there’s no effect in the population.” On the other hand, the alternative hypothesis (H A ) answers “Yes, there is an effect in the population.”

The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample.

You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.

The null hypothesis is the claim that there’s no effect in the population.

If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept. Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect”, “no difference”, or “no relationship”. When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .

The alternative hypothesis (H A ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.

Alternative hypotheses often include phrases such as “an effect”, “a difference”, or “a relationship”. When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes > or <). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.

Null and alternative hypotheses are similar in some ways:

• They’re both answers to the research question
• They both make claims about the population
• They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.

To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.

The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:

Does independent variable affect dependent variable ?

• Null hypothesis (H 0 ): Independent variable does not affect dependent variable .
• Alternative hypothesis (H A ): Independent variable affects dependent variable .

Test-specific

Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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• Knowledge Base

Methodology

• How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

• An independent variable is something the researcher changes or controls.
• A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

• H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
• H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

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• Simple random sampling
• Stratified sampling
• Cluster sampling
• Likert scales
• Reproducibility

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Research bias

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A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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9.1 Null and Alternative Hypotheses

The actual test begins by considering two hypotheses . They are called the null hypothesis and the alternative hypothesis . These hypotheses contain opposing viewpoints.

H 0 , the — null hypothesis: a statement of no difference between sample means or proportions or no difference between a sample mean or proportion and a population mean or proportion. In other words, the difference equals 0.

H a —, the alternative hypothesis: a claim about the population that is contradictory to H 0 and what we conclude when we reject H 0 .

Since the null and alternative hypotheses are contradictory, you must examine evidence to decide if you have enough evidence to reject the null hypothesis or not. The evidence is in the form of sample data.

After you have determined which hypothesis the sample supports, you make a decision. There are two options for a decision. They are reject H 0 if the sample information favors the alternative hypothesis or do not reject H 0 or decline to reject H 0 if the sample information is insufficient to reject the null hypothesis.

Mathematical Symbols Used in H 0 and H a :

H 0 always has a symbol with an equal in it. H a never has a symbol with an equal in it. The choice of symbol depends on the wording of the hypothesis test. However, be aware that many researchers use = in the null hypothesis, even with > or < as the symbol in the alternative hypothesis. This practice is acceptable because we only make the decision to reject or not reject the null hypothesis.

Example 9.1

H 0 : No more than 30 percent of the registered voters in Santa Clara County voted in the primary election. p ≤ 30 H a : More than 30 percent of the registered voters in Santa Clara County voted in the primary election. p > 30

A medical trial is conducted to test whether or not a new medicine reduces cholesterol by 25 percent. State the null and alternative hypotheses.

Example 9.2

We want to test whether the mean GPA of students in American colleges is different from 2.0 (out of 4.0). The null and alternative hypotheses are the following: H 0 : μ = 2.0 H a : μ ≠ 2.0

We want to test whether the mean height of eighth graders is 66 inches. State the null and alternative hypotheses. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

• H 0 : μ __ 66
• H a : μ __ 66

Example 9.3

We want to test if college students take fewer than five years to graduate from college, on the average. The null and alternative hypotheses are the following: H 0 : μ ≥ 5 H a : μ < 5

We want to test if it takes fewer than 45 minutes to teach a lesson plan. State the null and alternative hypotheses. Fill in the correct symbol ( =, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

• H 0 : μ __ 45
• H a : μ __ 45

Example 9.4

An article on school standards stated that about half of all students in France, Germany, and Israel take advanced placement exams and a third of the students pass. The same article stated that 6.6 percent of U.S. students take advanced placement exams and 4.4 percent pass. Test if the percentage of U.S. students who take advanced placement exams is more than 6.6 percent. State the null and alternative hypotheses. H 0 : p ≤ 0.066 H a : p > 0.066

On a state driver’s test, about 40 percent pass the test on the first try. We want to test if more than 40 percent pass on the first try. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

• H 0 : p __ 0.40
• H a : p __ 0.40

Collaborative Exercise

Bring to class a newspaper, some news magazines, and some internet articles. In groups, find articles from which your group can write null and alternative hypotheses. Discuss your hypotheses with the rest of the class.

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5.2 - writing hypotheses.

The first step in conducting a hypothesis test is to write the hypothesis statements that are going to be tested. For each test you will have a null hypothesis (\(H_0\)) and an alternative hypothesis (\(H_a\)).

When writing hypotheses there are three things that we need to know: (1) the parameter that we are testing (2) the direction of the test (non-directional, right-tailed or left-tailed), and (3) the value of the hypothesized parameter.

• At this point we can write hypotheses for a single mean (\(\mu\)), paired means(\(\mu_d\)), a single proportion (\(p\)), the difference between two independent means (\(\mu_1-\mu_2\)), the difference between two proportions (\(p_1-p_2\)), a simple linear regression slope (\(\beta\)), and a correlation (\(\rho\)). 
• The research question will give us the information necessary to determine if the test is two-tailed (e.g., "different from," "not equal to"), right-tailed (e.g., "greater than," "more than"), or left-tailed (e.g., "less than," "fewer than").
• The research question will also give us the hypothesized parameter value. This is the number that goes in the hypothesis statements (i.e., \(\mu_0\) and \(p_0\)). For the difference between two groups, regression, and correlation, this value is typically 0.

Hypotheses are always written in terms of population parameters (e.g., \(p\) and \(\mu\)).  The tables below display all of the possible hypotheses for the parameters that we have learned thus far. Note that the null hypothesis always includes the equality (i.e., =).

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Null Hypothesis Examples

The null hypothesis (H 0 ) is the hypothesis that states there is no statistical difference between two sample sets. In other words, it assumes the independent variable does not have an effect on the dependent variable in a scientific experiment .

The null hypothesis is the most powerful type of hypothesis in the scientific method because it’s the easiest one to test with a high confidence level using statistics. If the null hypothesis is accepted, then it’s evidence any observed differences between two experiment groups are due to random chance. If the null hypothesis is rejected, then it’s strong evidence there is a true difference between test sets or that the independent variable affects the dependent variable.

• The null hypothesis is a nullifiable hypothesis. A researcher seeks to reject it because this result strongly indicates observed differences are real and not just due to chance.
• The null hypothesis may be accepted or rejected, but not proven. There is always a level of confidence in the outcome.

What Is the Null Hypothesis?

The null hypothesis is written as H 0 , which is read as H-zero, H-nought, or H-null. It is associated with another hypothesis, called the alternate or alternative hypothesis H A or H 1 . When the null hypothesis and alternate hypothesis are written mathematically, they cover all possible outcomes of an experiment.

An experimenter tests the null hypothesis with a statistical analysis called a significance test. The significance test determines the likelihood that the results of the test are not due to chance. Usually, a researcher uses a confidence level of 95% or 99% (p-value of 0.05 or 0.01). But, even if the confidence in the test is high, there is always a small chance the outcome is incorrect. This means you can’t prove a null hypothesis. It’s also a good reason why it’s important to repeat experiments.

Exact and Inexact Null Hypothesis

The most common type of null hypothesis assumes no difference between two samples or groups or no measurable effect of a treatment. This is the exact hypothesis . If you’re asked to state a null hypothesis for a science class, this is the one to write. It is the easiest type of hypothesis to test and is the only one accepted for certain types of analysis. Examples include:

There is no difference between two groups H 0 : μ 1  = μ 2 (where H 0  = the null hypothesis, μ 1  = the mean of population 1, and μ 2  = the mean of population 2)

Both groups have value of 100 (or any number or quality) H 0 : μ = 100

However, sometimes a researcher may test an inexact hypothesis . This type of hypothesis specifies ranges or intervals. Examples include:

Recovery time from a treatment is the same or worse than a placebo: H 0 : μ ≥ placebo time

There is a 5% or less difference between two groups: H 0 : 95 ≤ μ ≤ 105

An inexact hypothesis offers “directionality” about a phenomenon. For example, an exact hypothesis can indicate whether or not a treatment has an effect, while an inexact hypothesis can tell whether an effect is positive of negative. However, an inexact hypothesis may be harder to test and some scientists and statisticians disagree about whether it’s a true null hypothesis .

How to State the Null Hypothesis

To state the null hypothesis, first state what you expect the experiment to show. Then, rephrase the statement in a form that assumes there is no relationship between the variables or that a treatment has no effect.

Example: A researcher tests whether a new drug speeds recovery time from a certain disease. The average recovery time without treatment is 3 weeks.

• State the goal of the experiment: “I hope the average recovery time with the new drug will be less than 3 weeks.”
• Rephrase the hypothesis to assume the treatment has no effect: “If the drug doesn’t shorten recovery time, then the average time will be 3 weeks or longer.” Mathematically: H 0 : μ ≥ 3

This null hypothesis (inexact hypothesis) covers both the scenario in which the drug has no effect and the one in which the drugs makes the recovery time longer. The alternate hypothesis is that average recovery time will be less than three weeks:

H A : μ < 3

Of course, the researcher could test the no-effect hypothesis (exact null hypothesis): H 0 : μ = 3

The danger of testing this hypothesis is that rejecting it only implies the drug affected recovery time (not whether it made it better or worse). This is because the alternate hypothesis is:

H A : μ ≠ 3 (which includes μ <3 and μ >3)

Even though the no-effect null hypothesis yields less information, it’s used because it’s easier to test using statistics. Basically, testing whether something is unchanged/changed is easier than trying to quantify the nature of the change.

Remember, a researcher hopes to reject the null hypothesis because this supports the alternate hypothesis. Also, be sure the null and alternate hypothesis cover all outcomes. Finally, remember a simple true/false, equal/unequal, yes/no exact hypothesis is easier to test than a more complex inexact hypothesis.

• Adèr, H. J.; Mellenbergh, G. J. & Hand, D. J. (2007).  Advising on Research Methods: A Consultant’s Companion . Huizen, The Netherlands: Johannes van Kessel Publishing. ISBN  978-90-79418-01-5 .
• Cox, D. R. (2006).  Principles of Statistical Inference . Cambridge University Press. ISBN  978-0-521-68567-2 .
• Everitt, Brian (1998).  The Cambridge Dictionary of Statistics . Cambridge, UK New York: Cambridge University Press. ISBN 978-0521593465.
• Weiss, Neil A. (1999).  Introductory Statistics  (5th ed.). ISBN 9780201598773.

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Booth, T., Doumas, A., Murray, A.L. (2020). Null Hypothesis. In: Zeigler-Hill, V., Shackelford, T.K. (eds) Encyclopedia of Personality and Individual Differences. Springer, Cham. https://doi.org/10.1007/978-3-319-24612-3_1335

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Once you have developed a clear and focused research question or set of research questions, you’ll be ready to conduct further research, a literature review, on the topic to help you make an educated guess about the answer to your question(s). This educated guess is called a hypothesis.

In research, there are two types of hypotheses: null and alternative. They work as a complementary pair, each stating that the other is wrong.

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• Alternative Hypothesis (H a ) – This is also known as the claim. This hypothesis should state what you expect the data to show, based on your research on the topic. This is your answer to your research question.

Null Hypothesis:   H 0 : There is no difference in the salary of factory workers based on gender. Alternative Hypothesis :  H a : Male factory workers have a higher salary than female factory workers.

Null Hypothesis :  H 0 : There is no relationship between height and shoe size. Alternative Hypothesis :  H a : There is a positive relationship between height and shoe size.

Null Hypothesis :  H 0 : Experience on the job has no impact on the quality of a brick mason’s work. Alternative Hypothesis :  H a : The quality of a brick mason’s work is influenced by on-the-job experience.

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Video transcript

Hypothesis Testing

When you conduct a piece of quantitative research, you are inevitably attempting to answer a research question or hypothesis that you have set. One method of evaluating this research question is via a process called hypothesis testing , which is sometimes also referred to as significance testing . Since there are many facets to hypothesis testing, we start with the example we refer to throughout this guide.

An example of a lecturer's dilemma

Two statistics lecturers, Sarah and Mike, think that they use the best method to teach their students. Each lecturer has 50 statistics students who are studying a graduate degree in management. In Sarah's class, students have to attend one lecture and one seminar class every week, whilst in Mike's class students only have to attend one lecture. Sarah thinks that seminars, in addition to lectures, are an important teaching method in statistics, whilst Mike believes that lectures are sufficient by themselves and thinks that students are better off solving problems by themselves in their own time. This is the first year that Sarah has given seminars, but since they take up a lot of her time, she wants to make sure that she is not wasting her time and that seminars improve her students' performance.

The research hypothesis

The first step in hypothesis testing is to set a research hypothesis. In Sarah and Mike's study, the aim is to examine the effect that two different teaching methods – providing both lectures and seminar classes (Sarah), and providing lectures by themselves (Mike) – had on the performance of Sarah's 50 students and Mike's 50 students. More specifically, they want to determine whether performance is different between the two different teaching methods. Whilst Mike is skeptical about the effectiveness of seminars, Sarah clearly believes that giving seminars in addition to lectures helps her students do better than those in Mike's class. This leads to the following research hypothesis:

Before moving onto the second step of the hypothesis testing process, we need to take you on a brief detour to explain why you need to run hypothesis testing at all. This is explained next.

Sample to population

If you have measured individuals (or any other type of "object") in a study and want to understand differences (or any other type of effect), you can simply summarize the data you have collected. For example, if Sarah and Mike wanted to know which teaching method was the best, they could simply compare the performance achieved by the two groups of students – the group of students that took lectures and seminar classes, and the group of students that took lectures by themselves – and conclude that the best method was the teaching method which resulted in the highest performance. However, this is generally of only limited appeal because the conclusions could only apply to students in this study. However, if those students were representative of all statistics students on a graduate management degree, the study would have wider appeal.

In statistics terminology, the students in the study are the sample and the larger group they represent (i.e., all statistics students on a graduate management degree) is called the population . Given that the sample of statistics students in the study are representative of a larger population of statistics students, you can use hypothesis testing to understand whether any differences or effects discovered in the study exist in the population. In layman's terms, hypothesis testing is used to establish whether a research hypothesis extends beyond those individuals examined in a single study.

Another example could be taking a sample of 200 breast cancer sufferers in order to test a new drug that is designed to eradicate this type of cancer. As much as you are interested in helping these specific 200 cancer sufferers, your real goal is to establish that the drug works in the population (i.e., all breast cancer sufferers).

As such, by taking a hypothesis testing approach, Sarah and Mike want to generalize their results to a population rather than just the students in their sample. However, in order to use hypothesis testing, you need to re-state your research hypothesis as a null and alternative hypothesis. Before you can do this, it is best to consider the process/structure involved in hypothesis testing and what you are measuring. This structure is presented on the next page .

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• v.23(Suppl 3); 2019 Sep

An Introduction to Statistics: Understanding Hypothesis Testing and Statistical Errors

Priya ranganathan.

1 Department of Anesthesiology, Critical Care and Pain, Tata Memorial Hospital, Mumbai, Maharashtra, India

2 Department of Surgical Oncology, Tata Memorial Centre, Mumbai, Maharashtra, India

The second article in this series on biostatistics covers the concepts of sample, population, research hypotheses and statistical errors.

How to cite this article

Ranganathan P, Pramesh CS. An Introduction to Statistics: Understanding Hypothesis Testing and Statistical Errors. Indian J Crit Care Med 2019;23(Suppl 3):S230–S231.

Two papers quoted in this issue of the Indian Journal of Critical Care Medicine report. The results of studies aim to prove that a new intervention is better than (superior to) an existing treatment. In the ABLE study, the investigators wanted to show that transfusion of fresh red blood cells would be superior to standard-issue red cells in reducing 90-day mortality in ICU patients. 1 The PROPPR study was designed to prove that transfusion of a lower ratio of plasma and platelets to red cells would be superior to a higher ratio in decreasing 24-hour and 30-day mortality in critically ill patients. 2 These studies are known as superiority studies (as opposed to noninferiority or equivalence studies which will be discussed in a subsequent article).

SAMPLE VERSUS POPULATION

A sample represents a group of participants selected from the entire population. Since studies cannot be carried out on entire populations, researchers choose samples, which are representative of the population. This is similar to walking into a grocery store and examining a few grains of rice or wheat before purchasing an entire bag; we assume that the few grains that we select (the sample) are representative of the entire sack of grains (the population).

The results of the study are then extrapolated to generate inferences about the population. We do this using a process known as hypothesis testing. This means that the results of the study may not always be identical to the results we would expect to find in the population; i.e., there is the possibility that the study results may be erroneous.

HYPOTHESIS TESTING

A clinical trial begins with an assumption or belief, and then proceeds to either prove or disprove this assumption. In statistical terms, this belief or assumption is known as a hypothesis. Counterintuitively, what the researcher believes in (or is trying to prove) is called the “alternate” hypothesis, and the opposite is called the “null” hypothesis; every study has a null hypothesis and an alternate hypothesis. For superiority studies, the alternate hypothesis states that one treatment (usually the new or experimental treatment) is superior to the other; the null hypothesis states that there is no difference between the treatments (the treatments are equal). For example, in the ABLE study, we start by stating the null hypothesis—there is no difference in mortality between groups receiving fresh RBCs and standard-issue RBCs. We then state the alternate hypothesis—There is a difference between groups receiving fresh RBCs and standard-issue RBCs. It is important to note that we have stated that the groups are different, without specifying which group will be better than the other. This is known as a two-tailed hypothesis and it allows us to test for superiority on either side (using a two-sided test). This is because, when we start a study, we are not 100% certain that the new treatment can only be better than the standard treatment—it could be worse, and if it is so, the study should pick it up as well. One tailed hypothesis and one-sided statistical testing is done for non-inferiority studies, which will be discussed in a subsequent paper in this series.

STATISTICAL ERRORS

There are two possibilities to consider when interpreting the results of a superiority study. The first possibility is that there is truly no difference between the treatments but the study finds that they are different. This is called a Type-1 error or false-positive error or alpha error. This means falsely rejecting the null hypothesis.

The second possibility is that there is a difference between the treatments and the study does not pick up this difference. This is called a Type 2 error or false-negative error or beta error. This means falsely accepting the null hypothesis.

The power of the study is the ability to detect a difference between groups and is the converse of the beta error; i.e., power = 1-beta error. Alpha and beta errors are finalized when the protocol is written and form the basis for sample size calculation for the study. In an ideal world, we would not like any error in the results of our study; however, we would need to do the study in the entire population (infinite sample size) to be able to get a 0% alpha and beta error. These two errors enable us to do studies with realistic sample sizes, with the compromise that there is a small possibility that the results may not always reflect the truth. The basis for this will be discussed in a subsequent paper in this series dealing with sample size calculation.

Conventionally, type 1 or alpha error is set at 5%. This means, that at the end of the study, if there is a difference between groups, we want to be 95% certain that this is a true difference and allow only a 5% probability that this difference has occurred by chance (false positive). Type 2 or beta error is usually set between 10% and 20%; therefore, the power of the study is 90% or 80%. This means that if there is a difference between groups, we want to be 80% (or 90%) certain that the study will detect that difference. For example, in the ABLE study, sample size was calculated with a type 1 error of 5% (two-sided) and power of 90% (type 2 error of 10%) (1).

Table 1 gives a summary of the two types of statistical errors with an example

Statistical errors

In the next article in this series, we will look at the meaning and interpretation of ‘ p ’ value and confidence intervals for hypothesis testing.

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Conflict of interest: None

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Statistic consultant for academic research.

Quantitative Research Hypothesis Examples

In general, a researcher arranges hypotheses based on the formulation of problems and theoretical studies. For quantitative research, the hypothesis used is a statistical hypothesis, meaning that the hypothesis must be tested using statistical rules. Whereas for qualitative research does not need to use statistical rules. In a quantitative study, the formulated statistical hypothesis has two forms, the null hypothesis (Ho) and the alternative hypothesis (Ha). In general, hypotheses for quantitative research have three types: Descriptive Hypothesis, Comparative Hypothesis, and Associative Hypothesis.

Descriptive Hypothesis

Descriptive hypotheses are temporary conjectures about the value of a variable, not expressing relationships or comparisons. Remember, only about the value of a variable. Statistics used to test descriptive hypotheses are sample mean tests or standard deviation tests. A researcher formulates hypothesis based on the problem formulation and theoretical study. Following are some examples of problem formulations (PF), hypotheses (H). PF: What is the percentage of junior high school mathematics mastery in the subject matter of the set? H: Junior high school mathematics teacher mastery in the subject matter reaches 70%.

PF: How good is the grade XI mastery of class XI material? H: mastery of class X material by class XI students reaches 75%.

Comparative Hypothesis

The comparative hypothesis is a temporary construct that compares the values ​​of two variables. That is, in the comparative hypothesis, we do not determine with certainty the value of the variables we examine, but compare. Means, there are two variables that are the same, but different samples. The statistics used to test this comparative hypothesis are (assuming normality is met) using a t-test. But before that, the normality and homogeneity must be tested first. Following are some examples of problem formulations (PF), hypotheses (H). PF: Is there a difference in the problem-solving abilities of students who got X learning better than students who got Y learning? H: the problem solving ability of students who get learning X is better than students who get learning Y.

PF: Are there differences in the critical thinking skills of students who study during the day are better than students who study in the morning? H: there is no difference in the critical thinking skills of students who study in the afternoon with students who study in the morning.

The two hypothetical examples above are slightly different. In the first hypothesis, we claim that the problem solving ability of students who get learning X is better than students who get learning Y. While in the second hypothesis, there is no one-sided claim that the critical thinking skills of students who learn during the day are better or worse. We only state that there are differences. Which problem is better, it does not concern this hypothesis. The first hypothesis is a one-party test hypothesis, while the second hypothesis is called a two-party test hypothesis.

Associative Quantitative Hypothesis

The associative hypothesis is a relationship between the relationship between two variables, the dependent variable and the independent variable. The statistics are used to test this comparative hypothesis are (assuming normality is met) using Product Moment Correlation, Double Correlation, or Partial Correlation. The following are examples of problem formulations (PF), hypotheses (H). PF: Is there a relationship between student achievement and the level of student anxiety? H: there is a negative relationship between student achievement with the level of student anxiety.

PF: Is there a relationship between student learning outcomes and seating arrangements? H: there is a positive relationship between student achievement with the level of student anxiety. In the first hypothesis there are the words ‘negative relationship’. Negative relationship means inversely proportional. That is if the level of student anxiety is high, then student achievement is low. Whereas in the second hypothesis there are the words ‘positive relationship’. Positive relationship means directly proportional. It means if the seating arrangement is good, the student learning outcomes are high.

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7.3: The Research Hypothesis and the Null Hypothesis

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Hypotheses are predictions of expected findings.

The Research Hypothesis

A research hypothesis is a mathematical way of stating a research question.  A research hypothesis names the groups (we'll start with a sample and a population), what was measured, and which we think will have a higher mean.  The last one gives the research hypothesis a direction.  In other words, a research hypothesis should include:

• The name of the groups being compared.  This is sometimes considered the IV.
• What was measured.  This is the DV.
• Which group are we predicting will have the higher mean.  

There are two types of research hypotheses related to sample means and population means:  Directional Research Hypotheses and Non-Directional Research Hypotheses

Directional Research Hypothesis

If we expect our obtained sample mean to be above or below the other group's mean (the population mean, for example), we have a directional hypothesis. There are two options:

• Symbol:       \( \displaystyle \bar{X} > \mu \)
• (The mean of the sample is greater than than the mean of the population.)
• Symbol:     \( \displaystyle \bar{X} < \mu \)
• (The mean of the sample is less than than mean of the population.)

Example \(\PageIndex{1}\)

A study by Blackwell, Trzesniewski, and Dweck (2007) measured growth mindset and how long the junior high student participants spent on their math homework.  What’s a directional hypothesis for how scoring higher on growth mindset (compared to the population of junior high students) would be related to how long students spent on their homework?  Write this out in words and symbols.

Answer in Words:            Students who scored high on growth mindset would spend more time on their homework than the population of junior high students.

Answer in Symbols:         \( \displaystyle \bar{X} > \mu \) 

Non-Directional Research Hypothesis

A non-directional hypothesis states that the means will be different, but does not specify which will be higher.  In reality, there is rarely a situation in which we actually don't want one group to be higher than the other, so we will focus on directional research hypotheses.  There is only one option for a non-directional research hypothesis: "The sample mean differs from the population mean."  These types of research hypotheses don’t give a direction, the hypothesis doesn’t say which will be higher or lower.

A non-directional research hypothesis in symbols should look like this:    \( \displaystyle \bar{X} \neq \mu \) (The mean of the sample is not equal to the mean of the population).

Exercise \(\PageIndex{1}\)

What’s a non-directional hypothesis for how scoring higher on growth mindset higher on growth mindset (compared to the population of junior high students) would be related to how long students spent on their homework (Blackwell, Trzesniewski, & Dweck, 2007)?  Write this out in words and symbols.

Answer in Words:            Students who scored high on growth mindset would spend a different amount of time on their homework than the population of junior high students.

Answer in Symbols:        \( \displaystyle \bar{X} \neq \mu \) 

See how a non-directional research hypothesis doesn't really make sense?  The big issue is not if the two groups differ, but if one group seems to improve what was measured (if having a growth mindset leads to more time spent on math homework).  This textbook will only use directional research hypotheses because researchers almost always have a predicted direction (meaning that we almost always know which group we think will score higher).

The Null Hypothesis

The hypothesis that an apparent effect is due to chance is called the null hypothesis, written \(H_0\) (“H-naught”). We usually test this through comparing an experimental group to a comparison (control) group.  This null hypothesis can be written as:

\[\mathrm{H}_{0}: \bar{X} = \mu \nonumber \]

For most of this textbook, the null hypothesis is that the means of the two groups are similar.  Much later, the null hypothesis will be that there is no relationship between the two groups.  Either way, remember that a null hypothesis is always saying that nothing is different.  

This is where descriptive statistics diverge from inferential statistics.  We know what the value of \(\overline{\mathrm{X}}\) is – it’s not a mystery or a question, it is what we observed from the sample.  What we are using inferential statistics to do is infer whether this sample's descriptive statistics probably represents the population's descriptive statistics.  This is the null hypothesis, that the two groups are similar.  

Keep in mind that the null hypothesis is typically the opposite of the research hypothesis. A research hypothesis for the ESP example is that those in my sample who say that they have ESP would get more correct answers than the population would get correct, while the null hypothesis is that the average number correct for the two groups will be similar. 

In general, the null hypothesis is the idea that nothing is going on: there is no effect of our treatment, no relation between our variables, and no difference in our sample mean from what we expected about the population mean. This is always our baseline starting assumption, and it is what we seek to reject. If we are trying to treat depression, we want to find a difference in average symptoms between our treatment and control groups. If we are trying to predict job performance, we want to find a relation between conscientiousness and evaluation scores. However, until we have evidence against it, we must use the null hypothesis as our starting point.

In sum, the null hypothesis is always : There is no difference between the groups’ means OR There is no relationship between the variables .

In the next chapter, the null hypothesis is that there’s no difference between the sample mean   and population mean.  In other words:

• There is no mean difference between the sample and population.
• The mean of the sample is the same as the mean of a specific population.
• \(\mathrm{H}_{0}: \bar{X} = \mu \nonumber \)
• We expect our sample’s mean to be same as the population mean.

Exercise \(\PageIndex{2}\)

A study by Blackwell, Trzesniewski, and Dweck (2007) measured growth mindset and how long the junior high student participants spent on their math homework.  What’s the null hypothesis for scoring higher on growth mindset (compared to the population of junior high students) and how long students spent on their homework?  Write this out in words and symbols.

Answer in Words:            Students who scored high on growth mindset would spend a similar amount of time on their homework as the population of junior high students.

Answer in Symbols:    \( \bar{X} = \mu \)

Contributors and Attributions

Foster et al.  (University of Missouri-St. Louis, Rice University, & University of Houston, Downtown Campus)

Dr. MO ( Taft College )

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Quantitative Data Analysis

5 Hypothesis Testing in Quantitative Research

Mikaila Mariel Lemonik Arthur

Statistical reasoning is built on the assumption that data are normally distributed , meaning that they will be distributed in the shape of a bell curve as discussed in the chapter on Univariate Analysis . While real life often—perhaps even usually—does not resemble a bell curve, basic statistical analysis assumes that if all possible random samples from a population were drawn and the mean taken from each sample, the distribution of sample means, when plotted on a graph, would be normally distributed (this assumption is called the Central Limit Theorem ). Given this assumption, we can use the mathematical techniques developed for the study of probability to determine the likelihood that the relationships or patterns we observe in our data occurred due to random chance rather than due some actual real-world connection, which we call statistical significance.

Statistical significance is not the same as practical significance. The fact that we have determined that a given result is unlikely to have occurred due to random chance does not mean that this given result is important, that it matters, or that it is useful. Similarly, we might observe a relationship or result that is very important in practical terms, but that we cannot claim is statistically significant—perhaps because our sample size is too small, for instance. Such a result might have occurred by chance, but ignoring it might still be a mistake. Let’s consider some examples to make this a bit clearer. Assume we were interested in the impacts of diet on health outcomes and found the statistically significant result that people who eat a lot of citrus fruit end up having pinky fingernails that are, on average, 1.5 millimeters longer than those who tend not to eat any citrus fruit. Should anyone change their diet due to this finding? Probably not, even those it is statistically significant. On the other hand, if we found that the people who ate the diets highest in processed sugar died on average five years sooner than those who ate the least processed sugar, even in the absence of a statistically significant result we might want to advise that people consider limiting sugar in their diet. This latter result has more practical significance (lifespan matters more than the length of your pinky fingernail) as well as a larger effect size or association (5 years of life as opposed to 1.5 millimeters of length), a factor that will be discussed in the chapter on association .

While people generally use the shorthand of “the likelihood that the results occurred by chance” when talking about statistical significance, it is actually a bit more complicated than that. What statistical significance is really telling us is the likelihood (or probability ) that a result equal to or more “extreme [1] ” is true in the real world, rather than our results having occurred due to random chance or sampling error . Testing for statistical significance, then, requires us to understand something about probability.

A Brief Review of Probability

You might remember having studied probability in a math class, with questions about coin flips or drawing marbles out of a jar. Such exercises can make probability seem very abstract. But in reality, computations of probability are deeply important for a wide variety of activities, ranging from gambling and stock trading to weather forecasts and, yes, statistical significance.

Probability is represented as a proportion (or decimal number) somewhere between 0 and 1. At 0, there is absolutely no likelihood that the event or pattern of interest would occur; at 1, it is absolutely certain that the event or pattern of interest will occur. We indicate that we are talking about probability by using the symbol [latex]p[/latex]. For example, if something has a 50% chance of occurring, we would write [latex]p=0.5[/latex] or [latex]\frac {1}{2}[/latex]. If we want to represent the likelihood of something not occurring, we can write [latex]1-p[/latex].

Check your thinking: Assume you were flipping coins, and you called heads. The probability of getting heads on a coin flip using a fair coin (in other words, a normal coin that has not been weighted to bias the result) is 0.5. Thus, in 50% of coin flips you should get heads. Consider the following probability questions and write down your answers so you can check them against the discussion below.

• Imagine you have flipped the coin 29 times and you have gotten heads each time. What is the probability you will get heads on flip 30?
• What is the probability that you will get heads on all of the first five coin flips?
• What is the probability that you will get heads on at least one of the first five coin flips?

There are a few basic concepts from the mathematical study of probability that are important for beginner data analysts to know, and we will review them here.

Probability over Repeated Trials : The probability of the outcome of interest is the same in each trial or test, regardless of the results of the prior test. So, if we flip a coin 29 times and get heads each time, what happens when we flip it the 29th time? The probability of heads is still 0.5! The belief that “this time it must be tails because it has been heads so many times” or “this coin just wants to come up heads” is simply superstition, and—assuming a fair coin—the results of prior trials do not influence the results of this one.

Probability of Multiple Events : The probability that the outcome of interest will occur repeatedly across multiple trials is the product [2] of the probability of the outcome on each individual trial. This is called the multiplication theorem . Thinking about the multiplication theorem requires that we keep in mind the fact that when we multiply decimal numbers together, those numbers get smaller— thus, the probability that a series of outcomes will occur is smaller than the probability of any one of those outcomes occurring on its own. So, what is the probability that we will get heads on all five of our coin flips? Well, to figure that out, we need to multiply the probability of getting heads on each of our coin flips together. The math looks like this (and produces a very small probability indeed):

[latex]\frac {1}{2} \cdot \frac {1}{2} \cdot \frac {1}{2} \cdot \frac {1}{2} \cdot \frac {1}{2} = 0.03125[/latex]

Probability of One of Many Events : Determining the probability that the outcome of interest will occur on at least one out of a series of events or repeated trials is a little bit more complicated. Mathematicians use the addition theorem to refer to this, because the basic way to calculate it is to calculate the probability of each sequence of events (say, heads-heads-heads, heads-heads-tails, heads-tails-heads, and so on) and add them together. But the greater the number of repeated trials, the more complicated that gets, so there is a simpler way to do it. Consider that the probability of getting  no heads is the same as the probability of getting all tails (which would be the same as the probability of getting all heads that we calculated above). And the only circumstance in which we would not have at least one flip resulting in heads would be a circumstance in which all flips had resulted in tails. Therefore, what we need to do in order to calculate the probability that we get at least one heads is to subtract the probability that we get no heads from 1—and as you can imagine, this procedure shows us that the probability of the outcome of interest occurring at least once over repeated trials is higher than the probability of the occurrence on any given trial. The math would look like this:

[latex]1- (\frac{1}{2})^5=0.9688[/latex]

So why is this digression into the math of probability important? Well, when we test for statistical significance, what we are really doing is determining the probability that the outcome we observed—or one that is more extreme than that which we observed—occurred by chance. We perform this analysis via a procedure called Null Hypothesis Significance Testing.

Null Hypothesis Significance Testing

Null hypothesis significance testing , or NHST , is a method of testing for statistical significance by comparing observed data to the data we would expect to see if there were no relationship between the variables or phenomena in question. NHST can take a little while to wrap one’s head around, especially because it relies on a logic of double negatives: first, we state a hypothesis we believe not to be true (there is no relationship between the variables in question) and then, we look for evidence that disconfirms this hypothesis. In other words, we are assuming that there is no relationship between the variables—even though our research hypothesis states that we think there is a relationship—and then looking to see if there is any evidence to suggest there is not no relationship. Confusing, right?

So why do we use the null hypothesis significance testing approach?

• The null hypothesis—that there is no relationship between the variables we are exploring—would be what we would generally accept as true in the absence of other information,
• It means we are assuming that differences or patterns occur due to chance unless there is strong evidence to suggest otherwise,
• It provides a benchmark for comparing observed outcomes, and
• It means we are searching for evidence that disconforms our hypothesis, making it less likely that we will accept a conclusion that turns out to be untrue.

Thus, NHST helps us avoid making errors in our interpretation of the result. In particular, it helps us avoid Type 2 error , as discussed in the chapter on Bivariate Analyses . As a reminder, Type 2 error is error where you accept a hypothesis as true when in fact it was false (while Type 1 error is error where you reject the hypothesis when in fact it was true). For example, you are making a Type 1 error if you decide not to study for a test because you assume you are so bad at the subject that studying simply cannot help you, when in fact we know from research that studying does lead to higher grades. And you are making a Type 2 error if your boss tells you that she is going to promote you if you do enough overtime and you then work lots of overtime in response, when actually your boss is just trying to make you work more hours and already had someone else in mind to promote.

We can never remove all sources of error from our analyses, though larger sample sizes help reduce error. Looking at the formula for computing standard error , we can see that the standard error ([latex]SE[/latex]) would get smaller as the sample size ([latex]N[/latex]) gets larger. Note: σ is the symbol we use to represent standard deviation.

[latex]SE = \frac{\sigma}{\sqrt N}[/latex]

Besides making our samples larger, another thing that we can do is that we can choose whether we are more willing to accept Type 1 error or Type 2 error and adjust our strategies accordingly. In most research, we would prefer to accept more Type 1 error, because we are more willing to miss out on a finding than we are to make a finding that turns out later to be inaccurate (though, of course, lots of research does eventually turn out to be inaccurate).

Performing NHST

Performing NHST requires that our data meet several assumptions:

• Our sample must be a random sample—statistical significance testing and other inferential and explanatory statistical methods are generally not appropriate for non-random samples [3] —as well as representative and of a sufficient size (see the Central Limit Theorem above).
• Observations must be independent of other observations, or else additional statistical manipulation must be performed. For instance, a dataset of data about siblings would need to be handled differently due to the fact that siblings affect one another, so data on each person in the dataset is not truly independent.
• You must determine the rules for your significance test, including the level of uncertainty you are willing to accept (significance level) and whether or not you are interested in the direction of the result (one-tailed versus two-tailed tests, to be discussed below), in advance of performing any analysis.
• The number of significance tests you run should be limited, because the more tests you run, the greater the likelihood that one of your tests will result in an error. To make this more clear, if you are willing to accept a 5% probability that you will make the error of accepting a hypothesis as true when it is really false, and you run 20 tests, one of those tests (5% of them!) is pretty likely to have produced an incorrect result.

If our data has met these assumptions, we can move forward with the process of conducting an NHST. This requires us to make three decisions: determining our null hypothesis , our confidence level (or acceptable significance level), and whether we will conduct a one-tailed or a two-tailed test. In keeping with Assumption 3 above, we must make these decisions before performing our analysis. The null hypothesis is the hypothesis that there is no relationship between the variables in question. So, for example, if our research hypothesis was that people who spend more time with their friends are happier, our null hypothesis would be that there is no relationship between how much time people spend with their friends and their happiness.

Our confidence level is the level of risk we are willing to accept that our results could have occurred by chance. Typically, in social science research, researchers use p<0.05 (we are willing to accept up to a 5% risk that our results occurred by chance), p<0.01 (we are willing to accept up to a 1% risk that our results occurred by chance), and/or p<0.001 (we are willing to accept up to a 0.1% risk that our results occurred by chance). P, as was noted above, is the mathematical notation for probability, and that’s why we use a p-value to indicate the probability that our results may have occurred by chance. A higher p-value increases the likelihood that we will accept as accurate a result that really occurred by chance; a lower p-value increases the likelihood that we will assume a result occurred by chance when actually it was real. Remember, what the p-value tells us is not the probability that our own research hypothesis is true, but rather this: assuming that the null hypothesis is correct, what is the probability that the data we observed—or data more extreme than the data we observed—would have occurred by chance.

Whether we choose a one-tailed or a two-tailed test tells us what we mean when we say “data more extreme than.” Remember that normal curve? A two-tailed test is agnostic as to the direction of our results—and many of the most common tests for statistical significance that we perform, like the Chi square, are two-tailed by default. However, if you are only interested in a result that occurs in a particular direction, you might choose a one-tailed test. For instance, if you were testing a new blood pressure medication, you might only care if the blood pressure of those taking the medication is significantly lower than those not taking the medication—having blood pressure significantly higher would not be a good or helpful result, so you might not want to test for that.

Having determined the parameters for our analysis, we then compute our test of statistical significance. There are different tests of statistical significance for different variables (for example, the Chi square discussed in the chapter on bivariate analyses ), as you will see in other chapters of this text, but all of them produce results in a similar format. We then compare this result to the p value we already selected. If the p value produced by our analysis is lower than the confidence level we selected, we can reject the null hypothesis, as the probability that our result occurred by chance is very low. If, on the other hand, the p value produced by our analysis is higher than the confidence level we selected, we fail to reject the null hypothesis, as the probability that our result occurred by chance is too high to accept. Keep in mind this is what we do even when the p value produced by our analysis is quite close to the threshold we have selected. So, for instance, if we have selected the confidence level of p<0.05 and the p value produced by our analysis is p=0.0501, we still fail to reject the null hypothesis and proceed as if there is not any support for our research hypothesis.

Thus, the process of null hypothesis significance testing proceeds according to the following steps:

• Determine the null hypothesis
• Set the confidence level and whether this will be a one-tailed or two-tailed test
• Compute the test value for the appropriate significance test
• Compare the test value to the critical value of that test statistic for the confidence level you selected
• Determine whether or not to reject the null hypothesis

Your statistical analysis software will perform steps 3 and 4 for you (before there was computer software to do this, researchers had to do the calculations by hand and compare their results to figures on published tables of critical values). But you as the researcher must perform steps 1, 2, and 5 yourself.

Confidence Intervals & Margins of Error

When talking about statistical significance, some researchers also use the terms confidence intervals and margins of error . Confidence intervals are ranges of probabilities within which we can assume the true population parameter lies. Most typically, analysts aim for 95% confidence intervals, meaning that in 95 out of 100 cases, the population parameter will lie within the upper and lower levels specified by your confidence interval. These are calculated by your statistics software as well. The margin of error, then, is the range of values within the confidence interval. So, for instance, a 2021 survey of Americans conducted by the Robert Wood Johnson Foundation and the Harvard T.H. Chan School of Public Health found that 71% of respondents favor substantially increasing federal spending on public health programs. This poll had a 95% confidence interval with a +/- 3.6 margin of error. What this tells us is that there is a 95% probability (19 in 20) that between 67.4% (71-3.6) and 74.6% (71+3.6) of Americans favored increasing federal public health spending at the time the poll was conducted. When a figure reflects an overwhelming majority, such as this one, the margin of error may seem of little relevance. But consider a similar poll with the same margin of error that sought to predict support for a political candidate and found that 51.5% of people said they would vote for that candidate. In that case, we would have found that there was a 95% probability that between 47.9% and 55.1% of people intended to vote for the candidate—which means the race is total tossup and we really would have no idea what to expect. For some people, thinking in terms of confidence intervals and margins of error is easier to understand than thinking in terms of p values; confidence intervals and margins of error are more frequently used in analyses of polls while p values are found more often in academic research. But basically, both approaches are doing the same fundamental analysis—they are determining the likelihood that the results we observed or a similarly-meaningful result would have occurred by chance.

What Does Significance Testing Tell Us?

One of the most important things to remember about significance testing is that, while the word “significance” is used in ordinary speech to mean importance, significance testing does not tell us whether our results are important—or even whether they are interesting. A full understanding of the relationship between a given set of variables requires looking at statistical significance as well as association and the theoretical importance of the findings. Table 1 provides a perspective on using the combination of significance and association to determine how important the results of statistical analysis are—but even using Table 1 as a guide, evaluating findings based on theoretical importance remains key. So: make sure that when you are conducting analyses, you avoid being misled into assuming that significant results are sufficient for making broad claims about the importance and meaning of results. And remember as well that significance only tells us the likelihood that the pattern of relationships we observe occurred by chance—not whether that pattern is causal. For, after all, quantitative research can never eliminate all plausible alternative explanations for the phenomenon in question (one of the three elements of causation, along with association and temporal order).

• Getting 7 heads on 7 coin flips
• Getting 5 heads on 7 coin flips
• Getting 1 head on 10 coin flips

Then check your work using the Coin Flip Probability Calculator .

• As the advertised hourly pay for a job goes up, the number of job applicants increases.
• Teenagers who watch more hours of makeup tutorial videos on TikTok have, on average, lower self-esteem.
• Couples who share hobbies in common are less likely to get divorced.
• Assume a research conducted a study that found that people wearing green socks type on average one word per minute faster than people who are not wearing green socks, and that this study found a p value of p<0.01. Is this result statistically significant? Is this result practically significant? Explain your answers.
• If we conduct a political poll and have a 95% confidence interval and a margin of error of +/- 2.3%, what can we conclude about support for Candidate X if 49.3% of respondents tell us they will vote for Candidate X? If 24.7% do? If 52.1% do? If 83.7% do?
• One way to think about this is to imagine that your result has been plotted on a bell curve. Statistical significance tells us the probability that the "real" result—the thing that is true in the real world and not due to random chance—is at the same point as or further along the skinny tails of the bell curve than the result we have plotted. ↵
• In other words, what you get when you multiply. ↵
• They also are not appropriate for censuses—but you do not need inferential statistics in a census because you are looking at the entire population rather than a sample, so you can simply describe the relationships that do exist. ↵

A distribution of values that is symmetrical and bell-shaped.

A graph showing a normal distribution—one that is symmetrical with a rounded top that then falls away towards the extremes in the shape of a bell

The sum of all the values in a list divided by the number of such values.

The theorem that states that if you take a series of sufficiently large random samples from the population (replacing people back into the population so they can be reselected each time you draw a new sample), the distribution of the sample means will be approximately normally distributed.

A statistical measure that suggests that sample results can be generalized to the larger population, based on a low probability of having made a Type 1 error.

How likely something is to happen; also, a branch of mathematics concerned with investigating the likelihood of occurrences.

Measurement error created due to the fact that even properly-constructed random samples are do not have precisely the same characteristics as the larger population from which they were drawn.

The theorem in probability about the likelihood of a given outcome occurring repeatedly over multiple trials; this is determined by multiplying the probabilities together.

The theorem addressing the determination of the probability of a given outcome occurring at least once across a series of trials; it is determined by adding the probability of each possible series of outcomes together.

A method of testing for statistical significance in which an observed relationship, pattern, or figure is tested against a hypothesis that there is no relationship or pattern among the variables being tested

Null hypothesis significance testing.

The error you make when you do not infer a relationship exists in the larger population when it actually does exist; in other words, a false negative conclusion.

The error made if one infers that a relationship exists in a larger population when it does not really exist; in other words, a false positive error.

A measure of accuracy of sample statistics computed using the standard deviation of the sampling distribution.

The hypothesis that there is no relationship between the variables in question.

The probability that the sample statistics we observe holds true for the larger population.

A measure of statistical significance used in crosstabulation to determine the generalizability of results.

A range of estimates into which it is highly probable that an unknown population parameter falls.

A suggestion of how far away from the actual population parameter a sample statistic is likely to be.

Social Data Analysis Copyright © 2021 by Mikaila Mariel Lemonik Arthur is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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Neag School of Education

Educational Research Basics by Del Siegle

Null and alternative hypotheses.

Converting research questions to hypothesis is a simple task. Take the questions and make it a positive statement that says a relationship exists (correlation studies) or a difference exists between the groups (experiment study) and you have the alternative hypothesis. Write the statement such that a relationship does not exist or a difference does not exist and you have the null hypothesis. You can reverse the process if you have a hypothesis and wish to write a research question.

When you are comparing two groups, the groups are the independent variable. When you are testing whether something affects something else, the cause is the independent variable. The independent variable is the one you manipulate.

Teachers given higher pay will have more positive attitudes toward children than teachers given lower pay. The first step is to ask yourself “Are there two or more groups being compared?” The answer is “Yes.” What are the groups? Teachers who are given higher pay and teachers who are given lower pay. The independent variable is teacher pay. The dependent variable (the outcome) is attitude towards school.

You could also approach is another way. “Is something causing something else?” The answer is “Yes.”  What is causing what? Teacher pay is causing attitude towards school. Therefore, teacher pay is the independent variable (cause) and attitude towards school is the dependent variable (outcome).

By tradition, we try to disprove (reject) the null hypothesis. We can never prove a null hypothesis, because it is impossible to prove something does not exist. We can disprove something does not exist by finding an example of it. Therefore, in research we try to disprove the null hypothesis. When we do find that a relationship (or difference) exists then we reject the null and accept the alternative. If we do not find that a relationship (or difference) exists, we fail to reject the null hypothesis (and go with it). We never say we accept the null hypothesis because it is never possible to prove something does not exist. That is why we say that we failed to reject the null hypothesis, rather than we accepted it.

Del Siegle, Ph.D. Neag School of Education – University of Connecticut [email protected] www.delsiegle.com